The TMB PLUS2 is a ready-to-use, mono-component, one-step substrate for colorimetric detection in ELISAs based on Horseradish Peroxidase labeled antibodies. It contains 3, 3′, 5, 5′-tetramethylbenzidine, substrate buffer and hydrogen peroxide and offers approximately 40% higher activity level than TMB ONE, resulting in higher sensitivity and reduced incubation time.